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. 2014 Nov 13;10(11):e1004514. doi: 10.1371/journal.ppat.1004514

Figure 7. Lack of Cyclin A2 degradation promotes metaphase arrest, aberrant spindle formation and chromosomal instability in HCMV-infected cells.

Figure 7

Cells were infected with HCMV-wt, HCMV-UL21a-RXL2mut or HCMV-ΔUL21a. Infected cells were examined by immunofluorescence microscopy for localization and structural organization of chromosomal material (DAPI staining), centromeres (CENP-A staining) and mitotic spindles (α-Tubulin). Representative images are shown in (A). Chromosomes lacking centromeres and accordingly have lost spindle attachment are marked by arrowheads. Quantitative data based on at least 100 mitotic cells per sample are presented in (B). Non-mitotic cells were not included in this quantitative analysis.