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. 2014 Nov 13;10(11):e1004514. doi: 10.1371/journal.ppat.1004514

Table 1. Oligonucleotide primers used in this study.

Primer name Primer sequence (orientation 5′-3′, Pho: 5′ phosporylation)
UL21a-RRLFQmut-fw GCCCTTGCGCCGCTTGGCTTTCTACGCGCCGCGAGCTCGTGCGCGCGCCTTCCAGAATCATATACATCCTAGGGATAACAGGGTAATCGATTT
UL21a-RRLFQmut-rev CCAGCACTCGGCGCTGTTCTGGATGTATATGATTCTGGAAGGCGCGCGCACGAGCTCGCGGCGCGTAGAGCCAGTGTTACAACCAATTAACC
UL21a-RRLFQ-revert-fw GCCCTTGCGCCGCTTGGCTTTCTACGCGCCGCGAGCTCGTCGGAGGCTTTTCCAGAATCATATACATCCTAGGGATAACAGGGTAATCGATTT
UL21a-RRLFQ-revert-rev CCAGCACTCGGCGCTGTTCTGGATGTATATGATTCTGGAAAAGCCTCCGACGAGCTCGCGGCGCGTAGAGCCAGTGTTACAACCAATTAACC
UL21a-PRmutAA-fw TGTTCCCCCCCATGTACCCGGTTTTGCTCCCTACCGCGTCGCCGCTCCCCACCCCATGATTCCCGATAGGGATAACAGGGTAATCGATTT
UL21a-PRmutAA-rev AAAACTGGTCCCAATGTTCTTCGGGAATCATGGGGTGGGGAGCGGCGACGCGGTAGGGAGCAAAACGCCAGTGTTACAACCAATTAACC
UL21a-del-fw AGTCATGCAGCGTGCGGCGCCTCTCTCATGGATCCACTGTCACCGTCGCGTAGGGATAACAGGGTAATCGATTT
UL21a-del-rev ATATAGACTTTTATATGATCCCTGTACAGATGTAAATAAAATGTTTTTATCGCGACGGTGACAGTGGATCCATGAGAGAGGCGCCGCACGCTGCATGACTGCCAGTGTTACAACCAATTAACC
UL21a-fw-BamH1 CGTAGGATCCTATGGGAGGTAGCCCTGTTCC
UL21A-rev-Sal1 CGTAGTCGACAAACTGGTCCCAATGTTCTTC
UL21a-RXL1-ARAAF-BssH Pho-GCTCGCCGGCCCTTGGCGCGCGCGGCTTTCTACGCGCC
UL21a-RXL1-rev Pho-CCGAAAGTCCATGCGCACAGATGG
UL21a-RXL2-ARAFQ-BssH Pho-GCGCCGCGAGCTCGTGCGCGCGCCTTCCAGAATCATATAC
UL21a-RXL2-rev Pho-GTAGAAAGCCAAGCGGCGCAAGGG
UL21a-APC-AA-NarI Pho-GCTCCCTACCGCGTCGCGGCGCCCCACCCCATGATTC
UL21a-APC-rev Pho-AAAACCGGGTACATGAGGTGGAACATCGTCCAGC
CyclinA2-fw AACAGCCAGACATCACTAACA G
CyclinA2-rev TCAAACTTTGAGGCTAACAGCA
APC5-fw AACGATTTCCGCCTAATAGCT
APC5-rev AACCGCTTTCCTATAAACACC
GAPDH-fw TTCACCACCATGGAGAAG
GAPDH-rev CACACCCATCACAAACATGG