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. 2014 Apr 15;120(14):2130–2141. doi: 10.1002/cncr.28668

Figure 2.

Figure 2

The effect of PRL-3 on FLT3-ITD–negative AML cell proliferation. (A,B) Detection of the ectopic GFP-PRL-3 expression in U937 cells (A) and ML-1 cells (B) stably transduced with either pLVX-puro-EGFP-PRL-3 wt or EGFP-PRL-3 (C104S) expression constructs by western blotting. (C) Depletion of endogenous PRL-3 expression in ML-1 cells transfected by either 2 PRL-3–specific shRNAs (shPRL-3-1 and shPRL-3-2), respectively, or by control (Ctrl) shRNA by western blotting. (D,E) The effects of PRL-3 overexpression on the proliferation of U937 (D) and ML-1 cells (E) assayed by CCK8 cell proliferation methods. (F) The effects of endogenous PRL-3 depletion on ML-1 cell proliferation by CCK8 cell proliferation methods. (G-I) Colony formation of ML-1 (G) and U937 (H) cells transfected with either PRL-3 or its mutant, or ML-1 cells (I) with endogenous PRL-3 silencing by specific shRNA1 and shRNA2. Data were shown as mean ± standrad deviation of triplicate experiments. *P < .05; **P < .01.