Skip to main content
. 2014 Sep 12;56(7):499–510. doi: 10.1111/dgd.12149

Figure 1.

Figure 1

CRISPR/Cas9-mediated mutations of Hox genes in Ciona intestinalis. (A) Cel-I assay of polymerase chain reaction (PCR) amplifications that contained the target site of Hox3-sg3. PCR bands were treated with Cel-I prior to electrophoresis. The “Hox3-sg3+Cas9” lane indicates the PCR product derived from larvae into which 3.0 pg of Hox3-sg3 RNA and 15 pg of Cas9 mRNA were microinjected. “Uninjected” lane indicates the PCR product derived from uninjected control larvae. M, marker lane. The arrow indicates the position of Cel-I cleaved band. (B) Cel-I assay of PCR amplifications that contained the target site of Hox5-sg1. 3.0 pg of Hox5-sg1 RNA and 15 pg of Cas9 mRNA were microinjected. (C) Cel-I assays of PCR amplifications that contained the target sites of Hox12-sg2 and Hox12-sg3. 3.0 pg of a sgRNA and 15 pg of Cas9 mRNA were microinjected. In both cases, Cel-I sensitive bands were not detected.