Table 2.
Mutation frequencies induced by Crispr/Cas9 system
| Name of sgRNA | Quantity of injected RNA | Mutation frequency (in %) | n† | |
|---|---|---|---|---|
| sgRNA (in pg) | Cas9 mRNA (in pg) | |||
| Hox5-sg1 | 0.3 | 3.0 | Cel-I negative | — |
| 0.3 | 15 | Cel-I negative | — | |
| 1.5 | 3.0 | Cel-I negative | — | |
| 3.0 | 3.0 | Cel-I negative | — | |
| 1.5 | 15 | 40.0 | 20 | |
| 3.0 | 15 | 75.9 | 31 | |
| 3.0 | 30 | 62.8 | 35 | |
| Hox3-sg3 | 0.3 | 15 | Cel-I negative | — |
| 3.0 | 3.0 | Cel-I negative | — | |
| 1.5 | 15 | 54.5 | 22 | |
| 3.0 | 15 | 58.0 | 31 | |
| 3.0 | 30 | 67.7 | 31 | |
†Number of sequenced polymerase chain reaction (PCR) clones. When the Cel-I assay did not yield cleaved band, sequenceing wan not performed.