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. 2014 Sep 12;56(7):499–510. doi: 10.1111/dgd.12149

Table 4.

Mutation frequency at the putative off-target sites of Hox3-sg3†

Name of sites Sequence of the target site in DNA‡ Mutation frequency (in %) n§
On-target GGCAGCCATAAGAGTCAACA 58.0 31
Off-target 1 GGCAcCCATAtcAGTCAACt 0 16
Off-target 2 GaCAaCCATAAGAGaCcACA 0 16
Off-target 3 tcCAGCCATAAGAGTCtAtA Not examined

†3.0 pg of sgRNA and 15 pg of Cas9 mRNA were microinjected. ‡Mismatched nucleotides are shown in lower case. §Number of sequenced clones. ¶The score is the same as that shown in Table2.