Reduced Dam activity in vivo and in vitro, leading to a reduction of N-methyladenine in GATC sequences in DNA [80] |
A mutator phenotype [7] |
A hyper-recombination phenotype [259] |
Alleviation of EcoK restriction [264] |
Increased number of single-strand DNA strand breaks in dam lig (DNA ligase) cells [41] |
Increased number of double-strand breaks in a dam recBC strain [107] |
Increased sensitivity to UV light and certain chemicals [7, 109, 230, 255, 265–267] |
Increased drug-induced mutagenesis [97] |
Derepression of certain genes in the SOS regulon [110, 268] |
Increased spontaneous induction of lysogenic phages [38, 241] |
Inviability of dam mutant cells with mutations in recA, recB, recC, lexA, polA, priA or ruv [7, 107, 112, 268] |
Increased precise excision and transposition of Tnl0 and other transposons [269] |
Altered expression of certain chromosomal and non-chromosomal genes such as trpS, sulA, glnS, mom, dnaA, pap, traJ, finP, and tsp [4, 202, 205, 206] |
Suppression of some dam phenotypes by second-site mutation in mutS, mutH, and mutL [99, 100] |
Control of phage P1 DNA packaging into virions [219] |
Asynchronous initiation of chromosome DNA replication [134] |
Failure to support the growth of plasmids containing the E. coli origin (oriC) of chromosomal replication [270, 271] or the phage P1 ori [272] or those with the RepI replication protein [273] |
Failure of Dam methylated plasmids to transform dam mutants at high efficiency [16] |