Figure 6. IL-1α acts via the IL-1R and MyD88.
Endometrial epithelial and stromal cells were cultured in medium with vehicle or transfected with siRNA targeting (a) IL1R1 or (b) MYD88, or with a scramble siRNA sequence, followed by treatment with control medium or medium containing 10 ng/ml IL-1α for 24 h. Supernatants were collected and the concentrations of IL-6 measured by ELISA. Data are presented as mean + SEM of 4 independent experiments. Data were analyzed by GLM ANOVA, using the Bonferroni post hoc test; values differ, *** p < 0.001, ** p < 0.01, ND = not detected.