Table 1. Summary of the results from the electrochemical analysis. All potentials reported were measured against the Ag/AgCl electrode.
PySSCou | Cys | DTT | GSH | Hcy | |
---|---|---|---|---|---|
Eox [V] | −0.590 | −0.869 | 0.360 | −0.565 | −0.589 |
Ered [V] | −1.010 | −1.080 | −3.690 | −1.050 | −1.030 |
Eo1/2 [V] | −0.805 | −0.975 | −1.665 | −0.808 | −0.810 |
EoCell [a] [V] | 0 | 0.170 | 0.860 | 0.003 | 0.005 |
ΔGo [b] [J/mol] | 0 | −2900 | −15000 | −51 | −87 |
a) Difference between the standard reduction potentials (Eo1/2) of PySSCou and respective thiol compounds. b) Estimated Gibbs free energy based on ΔGo = -nFEocell, where n is the number of electrons involved (2 electrons for disulfide exchange reaction) and F is Faraday's constant.