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. Author manuscript; available in PMC: 2015 May 12.
Published in final edited form as: Nat Commun. 2014 Nov 12;5:5390. doi: 10.1038/ncomms6390

Figure 7. Mice seek and return to the portion of the apparatus where they receive light activation of VTA projections from DR VGluT3 neurons.

Figure 7

(a) Diagrams showing experimental timeline, virus injection in DR of VGluT3∷Cre mice, and VTA light stimulation of DR VGluT3 inputs. (b) VGluT3-ChR2-eYFP mice (but not VGluT3-eYFP mice) sought out and spent more time in the chamber where VTA light stimulation was given during training. On the test day, VGluT3-ChR2-eYFP mice but not VGluT3-eYFP mice also returned to and spent more time in the chamber where light stimulation was given on the previous day (main effect group: F1,18=4.87, P< 0.05, three-way ANOVA). Relative time spent in each chamber is represented as mean + s.e.m. (n = 10 each group). Blue rectangles indicate light stimulation available in the laser-associated chamber. VGluT3-ChR2-eYFP mice showed preference for the laser-associated chamber on both training day and test day (* P< 0.05, Newman-Keuls post hoc test).