Fig. 7.

Activation of Pink-1 in SLOS cells. Immunostaining of Pink-1 and Parkin in SLOS and control cells. Panel A: representative images from immunostaining of Pink-1 in SLOS and control cells, red fluorescence for Pink-1 and blue fluorescence for nuclei, top row for control cells and the lower row for SLOS cells. Panel B: representative images from immunostaining of Parkin in SLOS and control cells, red fluorescence for Parkin and blue fluorescence for nuclei, top row for control cells and lower row for SLOS cells. Panel C: Western-blot of Pink-1 and Parkin for SLOS and control cells; β-actin was used as an internal control to normalize protein loading. Panel D: Image analysis of Pink-1 and Parkin immunostaining. The relative intensity of Pink-1 or Parkin was normalized by nuclei staining. Pink-1 was significantly increased in SLOS cells (n = 3, p < 0.05), while there was no significant change of the ratio of red/blue fluorescence in Parkin immunostaining.