Fig. 8.
Increased autophagy in cell models on SLOS neurons. (A) Increased expression of LC3B-II in neuronal stem cells obtained from the brains of Dhcr7 knockout mice. Mutational defects of one of the SLOS genotypes (Dhcr7Δ3–05/Δ3–5) were generated in mice using genetic engineering. Whole cell protein extracts from heterozygote (+/−) and homozygote (−/−) cells were subjected to immunoblotting with LC3B-II antibody. (B) Increased LC3B-II in Dhcr7-deficient Neuro2a cells. The cellular 7-DHC was monitored in control Neuro2a (0.21 ± 0.01 ng/μg protein) and in Dhcr7-deficient cells (33.3 ± 6.2 ng/μg protein). Increased LC3B-II in neuroblastoma cells in which Dhcr7 was silenced with siRNA.