The alignment of various HumD-like proteins was performed using MacVector 11.0.4. The HumD-like proteins include: STM2230.1c (S. typhimurium; NP_461173), SPAB_00769 (S. paratyphi; YP_001587027), P1 HumD (YP_006569), P7 HumD (ZP_07505015), pO111_2 HumD (YP_003238012), fKO2 HumD (YP_006603), N15 HumD (NP_046921) and Ent638_1055 (Enterobacter sp. 638; YP_001175788). Coloration indicates the degree of similarity to the consensus sequence. Blue indicates identity to the consensus, while green indicates an amino acid residue that is highly similar to the consensus. The degree of similarity to the consensus further decreases in this order of coloration: yellow, orange, pink and white.