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. Author manuscript; available in PMC: 2014 Nov 14.
Published in final edited form as: EcoSal Plus. 2012 Nov;5(1):10.1128/ecosalplus.7.2.2. doi: 10.1128/ecosalplus.7.2.2

Figure 8. Pol V and pol II can promote both error-free and error-prone TLS of AAF-dG.

Figure 8

Either pol V or pol III can insert a base opposite an N-Acetyl-2-Aminofluorene (AAF)-dG lesion and both do so in a relatively error-free manner (shown on the left hand side of the figure). Pol V can extend the primer-terminus leading to error free TLS of the AAF-dG adduct. Alternatively, when the AAF-dG lesion is positioned in the 3'-GCG sequence context, and pol II performs the extension step (right-hand side), −2 base-pair frameshifts occur (76, 162).