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. 2014 Jul 30;6(8):2076–2087. doi: 10.1093/gbe/evu164

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© The Author(s) 2014. Published by Oxford University Press on behalf of the Society for Molecular Biology and Evolution.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.

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Fig. 1.— — PFGE analysis shows that the type B neurotoxin gene is not located on the chromosome. (A) Stained PFGE gel in which intact chromosomal DNA is separated from extrachromosomal DNA (faint bands at bottom). Southern blot of same gel, (B) probed for type B neurotoxin gene; (C) reprobed for chromosomally located rRNA genes. Lanes 1, NCTC 11199; 2, Hobbs FT50; 3, Eklund 17B; 4, Eklund 2B; 5, Colworth BL151; 6, CDC 3875; 7, CDC 4627 U-1; 8, CDC 5900 A-T-A3; 9, 2129B; 10, CDC 5900; 11, Kapchunka B2; 12, Kapchunka B5; 13, CB-S-30E; 14, ATCC 17844; 15, IFR 05/020; 16, IFR 05/024; 17, IFR 05/025; 18, MDa10. Lanes 1 and 18 contain DNA from strains of Group I Clostridium botulinum type A (B). Bands in panel B marked with white symbols are of known size as determined from their DNA sequence: (_*), 47.7 kb; (+), 63 kb; (#), 60.6 kb. Horizontal dashes at right of panel C indicate approximate positions and size (kb) of whole-phage lambda size markers.