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. 2014 Oct 6;289(46):31708–31718. doi: 10.1074/jbc.M114.583393

FIGURE 3.

FIGURE 3.

Integration and oligomerization of various concentrations of Bax in the absence and presence of 50 pm tBid or Bid. A, TIRF images at various concentrations of Cy3-labeled Bax (upper panels) and with 50 pm Bid (middle panels) or with 50 pm tBid (lower panels) on lipid bilayers (93% DPPC and 7% cardiolipin). B, numbers of the fluorophore spots at different concentrations of Cy3-labeled Bax and with 50 pm Bid or tBid. Error bars show the S.D. of five independent experiments. *, p < 0.05 and ***, p < 0.001 between the data in the absence and the presence of tBid. C, distributions of photobleaching steps of the fluorophore spots on lipid bilayers (93% DPPC and 7% cardiolipin) incubated with different concentrations of Cy3-labeled Bax and with 50 pm Bid or tBid. D, TIRF images of 200 pm Cy3-labeled Bax with various concentrations of tBid on lipid bilayers (93% DPPC and 7% cardiolipin). E, distributions of photobleaching steps of the fluorophore spots on lipid bilayers (93% DPPC and 7% cardiolipin) incubated with 200 pm Cy3-labeled Bax with various concentrations of tBid.