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. 2014 Oct 6;289(46):31708–31718. doi: 10.1074/jbc.M114.583393

FIGURE 4.

FIGURE 4.

Integration of Bax on bilayers may be encouraged by Bid, Bim, or Bak BH3 domain peptide. A, TIRF images of 200 pm Cy3-labeled Bax incubated with BH3 peptide on lipid bilayers (93% DPPC and 7% cardiolipin). The panel from left to right shows the images of 200 pm Bax, 200 pm Bax with 50 pm Bid, 50 pm Bim, or 50 pm Bak BH3 domain peptide, respectively. B, fluorophore spots quantifying 200 pm Cy3-labeled Bax with or without BH3 peptide. Error bars show the S.D. of five independent experiments. ***, p < 0.001 between the data in the absence and the presence of BH3 peptide. C, histograms of photobleaching steps of Cy3-labeled Bax with different BH3 peptides.