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. 2014 Sep 28;289(46):31751–31764. doi: 10.1074/jbc.M114.591883

FIGURE 3.

FIGURE 3.

Polyadenylation of H3.1 mRNA is specific for arsenic exposure. Total RNA was extracted from arsenic- and Cr(VI)-transformed BEAS-2B clones, clones spontaneously grown in agar, and parental BEAS-2B cells. mRNA was then converted to cDNA using oligo(dT) primers. H3.1 polyadenylation was then measured by quantitative RT-PCR. The relative gene expression level was normalized to 18 S rRNA expression and is presented as -fold change to the level expressed in BEAS-2B clones and parental cells or spontaneous control clones. A, polyadenylation of H3.1 in control and arsenic-transformed clones. C1–C3 are spontaneous control clones, and As1–As4 are arsenic-transformed clones. B, polyadenylation of H3.1 in control and Cr(VI)-transformed clones. P is a parental BEAS-2B cell, C1 is a spontaneous control clone, and Cr1 and Cr2 are Cr(VI)-transformed BEAS-2B clones. Statistical significance was calculated using an unpaired, two-tailed t test with * indicating a p value less than 0.05 and ** indicating a p value less than 0.01. Error bars represent S.D.