TABLE 1.

Summary of K_d and anisotropy values

All values are the means of three independent experiments. The errors shown are the standard deviations. ND stands for not determined.

	Kda	Kib	rfreec	rboundd	Δrmaxd
eIF1-Fl
40 S	49 ± 6	105 ± 9	0.264 ± 0.010	0.319 ± 0.012	0.055 ± 0.003
40 S + 1A	12 ± 0.4	39 ± 5	0.262 ± 0.013	0.336 ± 0.004	0.074 ± 0.009
40 S + 3j	72 ± 9	166 ± 8	0.258 ± 0.010	0.318 ± 0.003	0.060 ± 0.008
40 S + 1A + 3j	17 ± 5	41 ± 1	0.255 ± 0.006	0.331 ± 0.007	0.076 ± 0.012
40 S + 3	≤10	ND	0.289 ± 0.005	0.328 ± 0.008	0.039 ± 0.004
40 S + TC	27 ± 7	ND	0.266 ± 0.005	0.340 ± 0.008	0.075 ± 0.006
40 S + 1A + TC	≤10	ND	0.283 ± 0.003	0.348 ± 0.008	0.065 ± 0.007
40 S + 5	52 ± 5	ND	0.252 ± 0.006	0.308 ± 0.001	0.056 ± 0.005
43 S	≤10	ND	0.298 ± 0.005	0.375 ± 0.003	0.077 ± 0.003
eIF1A-Fl
40 S	62 ± 5	61 ± 7	0.185 ± 0.002	0.240 ± 0.004	0.055 ± 0.001
40 S + 1	21 ± 2	23 ± 5	0.193 ± 0.003	0.250 ± 0.006	0.057 ± 0.003
40 S + 3j	230 ± 30	260 ± 60	0.190 ± 0.003	0.240 ± 0.002	0.050 ± 0.003
40 S + 1 + 3j	58 ± 3	53 ± 5	0.193 ± 0.005	0.244 ± 0.002	0.052 ± 0.006
40 S + 3	260 ± 30	ND	0.193 ± 0.003	0.245 ± 0.005	0.052 ± 0.007
40 S + 1 + 3	35 ± 3	ND	0.196 ± 0.008	0.237 ± 0.010	0.041 ± 0.003
40 S + TC	64 ± 5	ND	0.187 ± 0.006	0.221 ± 0.010	0.035 ± 0.006
40 S + 1 + TC	≤5	ND	0.212 ± 0.004	0.242 ± 0.003	0.030 ± 0.004
40 S + 5	55 ± 3	ND	0.194 ± 0.003	0.251 ± 0.003	0.056 ± 0.002
43 S	≤5	ND	0.205 ± 0.002	0.239 ± 0.002	0.033 ± 0.001
eIF3j-Fl
40 S	8.8 ± 1.2	14 ± 2	0.193 ± 0.002	0.299 ± 0.003	0.106 ± 0.005
40 S + 1	18 ± 2	20 ± 3	0.179 ± 0.004	0.277 ± 0.003	0.098 ± 0.002
40 S + 1A	85 ± 9	110 ± 7	0.190 ± 0.004	0.292 ± 0.012	0.102 ± 0.009
40 S + 1 + 1A	∼400f	∼400g	0.182 ± 0.012	0.277 ± 0.019	0.095 ± 0.007
40 S + 3Δj	≤5	ND	0.234 ± 0.006	0.306 ± 0.003	0.072 ± 0.009
40 S + 1 + 3Δj	≤5	ND	0.217 ± 0.009	0.314 ± 0.009	0.097 ± 0.003
40 S + 1A + 3Δj	≤5	ND	0.215 ± 0.009	0.324 ± 0.003	0.109 ± 0.011
40 S + 1 + 1A + 3Δj	11 ± 2	ND	0.226 ± 0.007	0.296 ± 0.001	0.071 ± 0.006
40 S + TC	120 ± 20	ND	0.194 ± 0.004	0.314 ± 0.008	0.120 ± 0.011
40 S + 5	7.0 ± 1.2	ND	0.187 ± 0.003	0.273 ± 0.008	0.086 ± 0.006
43 S	15 ± 4	ND	0.216 ± 0.004	0.283 ± 0.001	0.067 ± 0.003
3Δj	100 ± 15	ND	0.171 ± 0.005	0.201 ± 0.005	0.030 ± 0.005

^a Equilibrium dissociation constants determined by titrating the eIF-Fl with the 40 S subunit under the experimental condition.

^b Equilibrium dissociation constants determined by titrating the unlabeled wild-type protein to the preformed eIF-Fl and 40 S complex under the experimental condition.

^c Anisotropy of the indicated eIF-Fl prior to addition of 40 S subunits under the experimental conditions.

^d Anisotropy of the indicated eIF-Fl in the 40 S bound state.

^e Difference between r_free and r_bound, representing the maximum anisotropy change.

^f The value was estimated from a titration ranging from [40 S] = 0 to 1100 nm.

^g The value was calculated with using the estimate K_d value.