FIGURE 4.

SPR analysis of Evasin-4-Fc alanine mutants. CCL3, CCL5, and CCL8 suspended at 800 nm in running buffer were injected on Evasin-4-Fc coated on an anti-human Fc chip. To improve detection, chemokine NusA fusion proteins were used. Evasin-4-Fc wild type (black solid line) displayed a strong binding to the three chemokines tested whereas Evasin-4-Fc E16A (black dotted line) and Evasin-4-Fc Y19A (black dashed line) showed reduced binding, especially in the case of CCL5. The double mutant Evasin-4-Fc E16A Y19A (dashed and dotted line) has almost no detectable interaction with any of the chemokines tested. R.U., response units.