FIGURE 5.

Interaction between Evasin-4 and CCL5 mutants. A. Inhibition of chemokine-mediated chemotaxis of L1.2/CCR5 cells with Evasin-4. CCL5 or the indicated mutant was used at 10 nm to induce cell migration. Evasin-4 inhibits the activity of all tested mutants (⁴⁴AANA⁴⁷, ⁵⁵AAWVA⁵⁹, H23A, E26A, G32P, G32K, and E66A) except CCL5 T8P. Data are presented as the mean of three measurements ± S.E. B, CCL5 mutants suspended at 200 (CCL5 E26A) or 1000 nm (CCL5 T8P and CCL5 F12A, respectively) in running buffer were injected on coated Evasin-4. As expected from previous studies, the obligate tetramer CCL5 E26A (black solid line) displayed a strong binding to Evasin-4. By comparison, CCL5 T8P (black dotted line) and CCL5 F12A (black dashed line) showed a significantly reduced binding to the CKBP, even at high concentrations. RU, response units.