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. 2014 Sep 9;289(46):31856–31865. doi: 10.1074/jbc.M114.589093

FIGURE 2.

FIGURE 2.

Generation of mutant and transgenic flies for dMK2 and dIscU. A, a fly line containing EY11791 excisions was established to characterize deficiencies in dMK2. The promoter region and 5′ portion of the dMK2 transcript, including the first two exons, were deleted in line dMK25B. B, a fly line containing KG01476 excisions was established to characterize deficiencies in CG9836. C, the mRNA expression in dMK2−/− and dMK2 rescue flies. D and E, the mRNA level of the selected transgenic flies for both dIscU-S20A and dIscU-s20E rescue flies. Error bars reflect S.E.