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. 2014 Sep 9;289(46):31856–31865. doi: 10.1074/jbc.M114.589093

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© 2014 by The American Society for Biochemistry and Molecular Biology, Inc.

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FIGURE 3. — dMK2 interacts with and phosphorylates dIscU. A and B, FLAG-dMK2 was coexpressed with Myc-dIscU, and FLAG-dIscU was coexpressed with Myc-dMK2 in 293T cells. FLAG-tagged protein was immunoprecipitated using FLAG M2 beads 36 h after transfection. The cell lysates and immunoprecipitates were analyzed by Western blot. C, GST-dIscU was incubated with or without GST-dMK2 in a kinase buffer containing [³²P]ATP. Phosphorylation of dIscU was detected by autoradiography. D, alignment of dIscU (CG9836) with its homologs. Hs, Homo sapiens; Mm, Mus musculus; Ec, E. coli.