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Both dIscU-S20A and dMK2−/− have no role in regulating aconitase activity neither in mitochondrial nor in cytosol. A and C, mitochondrial aconitase (m-aconitase) activity was measured by the Bioxytech Aconitase-340 kit (Oxis Research). 750 μg of mitochondrial protein were used. mOD, milli optical density. B and D, cytoplasmic aconitase (c-aconitase) activity was measured by the Bioxytech Aconitase-340 kit (Oxis Research). 200 μg of protein were used. Error bars reflect S.E.
