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dMK2 regulates complex I activity just as dIscU-S20A mutant does. A–D, mitochondrial complex I activity was measured from different fly lines by complex I enzyme activity microplate assay kit (MitoSciences). mOD, milli optical density. E, UAS-dMK2 transgenic flies were crossed with da-Gal4 flies. The dMK2 mRNA level was then measured by RT-PCR. F, the complex I activity of dMK2-overexpressing flies was measured. Error bars reflect S.E.; star signifies p < 0.05.
