FIGURE 1.

Synthesis (A) and cytotoxicity evaluation (B–D) of novel biscoumarins toward HCC cells. A, scheme represents the synthesis of biscoumarins (R-aromatic/heteroaromatic group). Twenty-three new biscoumarin derivatives were designated as 3(a-w). The anti-proliferative effects of BIHC against HepG2 (B), HCCLM3 (C), and Hep3B (D) cells were determined by MTT assay and reported as the percentage of viable cells relative to the control. Values are means ± S.E. of three independent experiments. * indicates p value < 0.05.