FIGURE 2.

A, cyclic voltammetry traces of B. anthracis NrdI and of free FMN. Recorded under anaerobic conditions using a graphite electrode in 50 mm Tris-HCl, pH 7.6. [NrdI] = 180 μm, scan rate 60 mV s⁻¹ (a); [NrdI] = 180 μm, scan rate 100 mV s⁻¹ (b); [FMN] = 150 μm, scan rate 10 mV s⁻¹ (c). B, cyclic square wave voltammetry traces of B. anthracis NrdI (solid black line) and of free FMN (solid gray line). Recorded under anaerobic conditions using a gold electrode in 50 mm Tris-HCl, pH 7.6. a and a′ anodic and cathodic scans, respectively, of NrdI (180 μm); b and b′ anodic and cathodic scans respectively of free FMN (150 μm). Square wave voltammograms were obtained using a square wave frequency of 10 Hz, pulse height = 25 mV, pulse width = 50 ms, and step height = 15 mV.