FIGURE 6.

Calcium activates mTORC1 via the lysosome v-ATPase pathway. A and B, serum-starved N41/OX1R cells were treated with 50 μm orexin-A or 1 μm ionomycin (A) or 5 μm thapsigargin (TG; B) for 1 h in the absence or presence of 20 μm BAPTA/AM. C, serum-starved N41/OX1R cells were treated with thapsigargin or ionomycin in the absence or presence of 20 nm rapamycin. D, serum-starved N41/OX1R cells were treated with 1 μm ionomycin for 1 h in the absence or presence of 5 μm or 10 μm bafilomycin A1. E, serum-starved N41/OX1R cells were treated with 5 μm thapsigargin for 1 h in the absence or presence of 10 μm saliPhe or bafilomycin A1. F, 293T/OX1R cells were infected with control shGFP or shRagC lentivirus for 24 h. After hygromycin selection for 72 h, the infected cells were serum-starved for 24 h followed by ionomycin treatment for 1 h. The levels of S6K, RagC, and Raptor and phosphorylations of S6 and S6K were detected by Western blotting.