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. 2014 Oct 3;289(46):31983–31994. doi: 10.1074/jbc.M114.574699

FIGURE 2.

FIGURE 2.

The enhancement in P2X7 receptor-mediated dye uptake by MCD treatment is independent of pannexin-1 and cytoskeletal changes. A, neither carbenoxolone (+carb; 30 μm) nor pretreatment with the caspase inhibitor zVAD-FMK (+zVAD; 5 μm for 1 h) reduced human P2X7-mediated ethidium uptake following MCD treatment. (n = 6, p > 0.05). B, preincubation with (−)-blebbistatin (+bleb; 100 μm for 1 h), an inhibitor of non-muscle myosin 2, had no effect on the rate of mouse P2X7-mediated dye uptake, with or without MCD pretreatment (n = 12, p > 0.05). C, the actin-stabilizing agent jasplakinolide (+jasp; 30 nm for 1 h) had no effect on human P2X7-mediated dye uptake with or without MCD treatment (n = 4–8, p > 0.05). D, latrunculin-A (+lat; 5 μm for 30 min), an inhibitor of F-actin polymerization, had no effect on the initial rate of mouse P2X7-mediated dye uptake, with or without MCD treatment (n = 3–6, p > 0.05).