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. 2014 Sep 30;289(46):32030–32043. doi: 10.1074/jbc.M113.538223

FIGURE 5.

FIGURE 5.

Rab11a deficiency impairs MMP7 secretion in blastocysts. A, RT-PCR detected Rab11a and Rab11b in embryos at the four-cell, morula, and blastocyst stages. Mouse gut total RNAs served as a control. B, immunofluorescent staining for Cdx2 (green) and Rab11a (red) in blastocysts. Both trophectoderm cells (Cdx2+) and the inner cell mass (Cdx2−) express Rab11a. The arrow points to intense Rab11a signals at a cleavage furrow within the inner cell mass. Scale bars = 20 μm. C, immunofluorescent staining for MMP7 (green) and Rab11a (red) in wild-type embryos. Scale bars = 10 μm. Some MMP7+ vesicles were positive for Rab11a (arrows). D, MMP7 enzymatic kinetics were measured from the medium of cultured Rab11a+/+, Rab11a+/−, and Rab11a−/− hatching blastocysts. Bovine serum served as positive control. Values obtained from blank M16 medium were subtracted from all experimental values. E, accumulative MMP7 enzyme activities were averaged from the entire measuring duration. **, p < 0.01.