FIGURE 2.

ATase1 and ATase2 exist as homo- and heterodimers and are part of a high molecular mass complex. A–C, immunoblots showing co-IP of myc- and halo-tagged versions of ATase1 and ATase2 when co-expressed in the same cells. A, ATase1 can form homodimers. B, ATase2 can form homodimers. C, ATase1 and ATase2 can form heterodimers. A schematic view of each experiment is shown in the lower panels. D and E, affinity-purified ATase1 (D) and ATase2 (E) migrate with the molecular mass of 300–350 kDa under native conditions. IP, immunoprecipitation; Wb, Western blot.