FIGURE 7.

Density light-scattering experiments. Cuvettes were prepared with purified MNK (10 μm) and ManNAc or 2-acetylamino-2-deoxy-3-O-methyl-d-mannose (8a) at concentrations of 5 mm. All measurements have been performed at 21 °C. As a positive control, MNK was preincubated for 10 min at 45 °C to induce protein aggregation. The radius of the highest peak for each graph is shown. Data depicted represent the mean values obtained in three independent experiments (n = 3). 8a does not cause any protein aggregation under given experimental conditions.