TABLE 1.
Comparison of rate constants for 2-acetylamino-2-deoxy-3-O-methyl-m-mannose (8a) binding to covalently immobilized, His-tagged MNK
SPR-binding sensorgrams were fitted using a 1:1 Langmuir binding model. Data was obtained in three independent experiments (n = 3) at a temperature of 25 °C. For ManNAc and methyl 2-acetylamino-2-deoxy-3-O-methyl-α-d-mannopyranoside (5), no binding could have been observed under given experimental conditions (NA, data not available). ka, on rate; kd, off rate; KD, dissociation constant.
| ManNAc | 8a | 5 | ATP | ADP | UDP | |
|---|---|---|---|---|---|---|
| ka (m−1 s−1) | NA | 2.92 | NA | 2.48 | 2.76 | NA |
| kd (s−1) | NA | 2.25 × 10−3 | NA | 1.06 × 10−3 | 8.40 × 10−3 | NA |
| KD (μm) | NA | 755 | NA | 429 | 306 | NA |
| Χ2 | NA | 7.65 | NA | 19.20 | 18.87 | NA |