FIGURE 2.

Representative dot blot results. Aβ40 (a) and Aβ42 (b) were aggregated under three different conditions over a 10-day time period. The immunological reactivities of the aggregates from time 0, and the 3- and 10-day time points were then tested with the 23 mOC antibodies, along with 6E10 and 4G8. Results obtained with antibodies 6E10, 4G8, and mOC 3, 9, 23, 31, 76, and 87 are displayed as representative examples. The three aggregation conditions were as follows: Condition A, peptide resuspended in 100 mm NaOH and diluted in phosphate buffer; Condition B, peptide resuspended in HFIP and diluted in water; Condition C, peptide resuspended in 100 mm NaOH and diluted in HEPES/NaCl buffer.