FIGURE 3.

Complete dot blot data. Aβ40 (a) and Aβ42 (b) were aggregated under three different conditions over a 10-day time period. 1-μl aliquots were pipetted onto nitrocellulose membranes at time 0 and at the 3- and 10-day time points. The membranes were then probed with the 23 mOC antibodies, along with 6E10 and 4G8. The three aggregation conditions were as follows: Condition A, peptide resuspended in 100 mm NaOH and diluted in phosphate buffer; Condition B, peptide resuspended in HFIP and diluted in water; Condition C, peptide resuspended in 100 mm NaOH and diluted in HEPES/NaCl buffer.