FIGURE 7.

Dot blot assays of IAPP and α-synuclein aggregates. α-Synculein (left) and IAPP (right) were aggregated over a 6-day time period. The immunoreactivities of samples from time 0 and the 1-, 2-, and 4–6-day time points were tested using the 23 mOC antibodies in a dot blot assay. Antibodies that showed positive reactivity with the α-synuclein and IAPP preparations are shown. IAPP was resuspended in 100 mm NaOH and diluted in PBS, and α-synculein was resuspended in HFIP and diluted in water.