FIGURE 4.

Metabolites profiled by GC-MS before and after rat liver homogenates were incubated with labeled and unlabeled HNA. A, overlay of GC-MS chromatograms from liver homogenates incubated with 1 mm HNA in the presence of 1 mm NADPH at 0 and 90 min. The inset is an extended GC-MS chromatogram (long running method with better separation) of peak 1. B and C, EI mass spectra of peak I (4,8-DHNA, ω-1-hydroxylation product) when liver homogenates were incubated with unlabeled HNA and [3,4-¹³C₂]HNA. D and E, EI mass spectra of peak II (4,9-DHNA, ω-hydroxylation product) when liver homogenates were incubated with unlabeled HNA and [3,4-¹³C₂]HNA.