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. 2014 Oct 1;289(46):32327–32338. doi: 10.1074/jbc.M114.602458

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© 2014 by The American Society for Biochemistry and Molecular Biology, Inc.

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FIGURE 6. — 4,8-DHNA and 4,9-DHNA production in rat brain, heart, kidney, and liver homogenates incubated with HNA via ω-1- and ω-hydroxylation. A and B, 4,8-DHNA and 4,9-DHNA productions from HNA incubated in rat brain, heart, kidney, and liver homogenates. C, total amount of 4,8-DHNA and 4,9-DHNA production. D, ratio of 4,9-DHNA/4,8-DHNA. Rat organ homogenates were incubated with 1 mm HNA, 1 mm NADPH, and 100 mm phosphate buffer at 37 °C for 30 min. ω- and ω-1-hydroxylation products were assayed by GC-MS. Error bar, S.D. of measurements (n = 5).