FIGURE 2.

Amino acid and amino sugar composition of the material recovered in a spore coat isolation protocol. Wild type (wt; DK1622), ΔexoC (PH1261), wzm epsV (PH1285), and ΔnfsA-H (Δnfs; PH1200) cells were induced to sporulate for 4 h by addition of glycerol. Material was isolated and acid-hydrolyzed, and a sample volume corresponding to 0.2 mg of protein per lane was loaded on a cellulose TLC plate and stained with ninhydrin. Marker, 2 μl of 10 mm each glutamate, glycine, alanine, and galactosamine in water. Spots 1–3, which were further analyzed by mass spectrometry, are indicated to the right.