FIGURE 4.

Amino acid and amino sugar composition of spore coat material recovered from individual exo mutants. Wild type (wt; DK1622), ΔexoB (PH1303), ΔexoD (PH1304), ΔexoE (PH1265), ΔexoF (PH1507), ΔexoG (PH1508), ΔexoH (PH1264), ΔexoI (PH1511), ΔexoC (PH1261) and ΔnfsA-H (Δnfs; PH1200) cells were induced to sporulate for 4 h by addition of glycerol. Material was isolated and acid-hydrolyzed, and a sample volume corresponding to 0.2 mg of protein per lane was loaded on a cellulose TLC plate and stained with ninhydrin. Marker, 2 μl of 10 mm glutamate, glycine, alanine, and galactosamine in water.