TABLE 1.
Strains and plasmids used in this study
| Strain or plasmid | Genotype | Ref. or source |
|---|---|---|
| M. xanthus | ||
| DK1622 | Wild type | 59 |
| PH1261 | DK1622 ΔexoC | 5 |
| PH1200 | DK1622 ΔnfsA-H | 9 |
| PH1303 | DK1622 ΔexoB | This study |
| PH1304 | DK1622 ΔexoD | This study |
| PH1265 | DK1622 ΔexoE | This study |
| PH1507 | DK1622 ΔexoF | This study |
| PH1508 | DK1622 ΔexoG | This study |
| PH1264 | DK1622 ΔexoH | This study |
| PH1511 | DK1622 ΔexoI | This study |
| PH1509 | DK1622 ΔMXAN_1035 | This study |
| PH1270 | DK1622 wzm::ΩKanR | This study |
| PH1285 | DK1622 wzm::ΩKanR epsV::pCH19 | This study |
| PH1296 | DK1622 ΔexoC wzm::ΩKanR epsV::pCH19 | This study |
| HK1321 | DK1622 wzm::ΩKanR | 18, 60 |
| E. coli | ||
| TOP10 | Host for cloning [F− mcrA Δ(mrr-hsdRMS-mcrBC) Φ80lacZΔM15ΔlacX74 deoR recA1 arsD139 Δ(ara-leu)7697 galU halK rpsL (Strr) endA1 nupG] | Invitrogen |
| BL21(DE3) | Host for overexpression (fhuA2 [lon] ompT gal (λ DE3) [dcm] ΔhsdS) | Novagen |
| Plasmids | ||
| pBJ114 | Backbone for in-frame deletions; galK, KanR | 61 |
| pUC19 | cloning vector, AmpR | Thermo Scientific |
| pSW30 | Source of TcR cassette | 62 |
| pCDF-Duet | PrT7, SpcR | Novagen |
| pCOLA-Duet | PrT7, KanR | Novagen |
| pCH14–2 | pBJ114 ΔexoE (codons 5–451) | This study |
| pCH15–1 | pBJ114 ΔexoH (codons 11–374) | This study |
| pCH48 | pBJ114 ΔexoG (codons 16–337) | This study |
| pCH49 | pBJ114 ΔexoF (codons 26–351) | This study |
| pCH50 | pBJ114 ΔexoI (codons 8–381) | This study |
| pCH51 | pBJ114 ΔexoB (codons 16–388) | This study |
| pCH63 | pBJ114 ΔexoD (codons 7–215) | This study |
| pCH62 | pBJ114 ΔMXAN_1035 (codons 14–473) | This study |
| pCH19 | Internal fragments of epsV (codons 323–500) and TcR in pUC19 | This study |
| pCH20 | The nfsA–C coding region in pCDF-Duet | This study |
| pCH21 | The nfsD–H coding region in pCOLA-Duet | This study |
| pCH57 | The coding region of nfsC in pCDF-Duet | This study |