TABLE 4.
Glucose (Glc) and N-acetylgalactosamine (GalNAc) linkages identified in spore coat material isolated from wild type ΔnfsA–H (Δnfs) strains
Average and standard deviations of two independent analyses from independent biological replicates are shown. ND means none detected.
| Residue | Genotype (strain) |
|
|---|---|---|
| WT (DK1622) | Δnfs (PH1200) | |
| GalNAc | 100 | 100 |
| t-GalNAc | 41 ± 0 | 3 ± 1 |
| 4-GalNAc | 44 ± 3 | 62 ± 8 |
| 3-GalNAc | 15 ± 2 | 32 ± 5 |
| 3,4-GalNAc | ND | 1 ± 1 |
| 4,6-GalNAc | ND | 1 ± 2 |
| Glc | 100 | 100 |
| t-Glc | 26 ± 2 | 12 ± 3 |
| 6-Glc | 6 ± 3 | 2 ± 0 |
| 4-Glc | 64 ± 3 | 83 ± 6 |
| 3,4-Glc | 1 ± 2 | 1 ± 1 |
| 2,4-Glc | 1 ± 1 | 1 ± 1 |
| 4,6-Glc | 2 ± 0 | 1 ± 1 |
| 2,3,4,6-Glc | ND | 1 ± 1 |