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. Author manuscript; available in PMC: 2014 Nov 14.
Published in final edited form as: J Immunol. 2000 Jun 15;164(12):6503–6508. doi: 10.4049/jimmunol.164.12.6503

FIGURE 2.

FIGURE 2

15d-PGJ2 inhibits IP-10 mRNA and protein expression in a concentration-dependent manner. A, Representative Northern blot analysis of human ECs stimulated for 12 h with IFN-γ (100 U/ml) and 15d-PGJ2 at concentrations shown. GAPDH expression demonstrates equal loading of intact RNA (upper panel). Lower panel shows densitometry analysis of three independent Northern blot experiments. Results of IP-10 expression were normalized to GAPDH and are expressed as percent of IFN-γ-stimulated cells (% control). Bars represent mean ± SEM. **, p < 0.01 vs IFN-γ-stimulated cells. B, Western blot analysis of secreted IP-10 protein from human ECs. Cells were stimulated for 24 h with IFN-γ (100 U/ml) and 15d-PGJ2 at concentrations indicated before supernatants were harvested for analysis. Recombinant IP-10 protein served as a positive control.