Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2014 Sep 8;42(19):12138–12154. doi: 10.1093/nar/gku815

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2014. Published by Oxford University Press on behalf of Nucleic Acids Research.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.

PMC Copyright notice

Figure 2. — ChemModSeq accurately and quantitatively measures RNA secondary structure. (A) Primer extension results and ChemModSeq drop-off rates for DMS modified and unmodified 18S rRNA, focusing on the first ∼100 nucleotides. To compare ChemModSeq with primer extension data, drop-off rates were converted to gray scale heat maps in which each block represents a single nucleotide. The darker the color of the block, the higher the drop-off rate. Oligos used for primer extensions are indicated in bold (see Supplementary Table S4). The positions of the modified nucleotides are indicated on the right side of each panel. (B) ChemModSeq data agree well with primer extension results. Direct comparison of the primer extension results shown in (A), quantified using SAFA (34) (red bars) and ChemModSeq RT drop-off rates (blue bars) for the same region (nucleotides 31–98).