Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2014 Sep 27;42(19):12177–12188. doi: 10.1093/nar/gku863

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2014. Published by Oxford University Press on behalf of Nucleic Acids Research.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.

PMC Copyright notice

Figure 3. — Effects of RNA binding energy and structure stability on sRNA efficiency. For each sequence variant we compare the change in local RNA stability or target binding free energy (kcal/mol) to sRNA repression/activation of its targets. (A–F) Variants of RyhB, (G–J) variants of DsrA. Seed interactions (A, B, G–H) are modeled as binding free energy of the sRNA-mRNA duplex. (A) RyhB-sodB (R² = 0.62, N = 158), (B) RyhB-shiA (R² = 0.42, N = 277), (G) DsrA-hns (R² = 0.37, N = 35), (H) DsrA-rpoS (N = 60). Stem loop stability (C–F, I, J) is modeled by self-folding free energy. Stability of RyhB SL1 compared with regulation of (C) sodB (R² = 0.46, N = 1223) and (D) shiA (R² = 0.13, N = 792); stability of RyhB SL3 and regulation of (E) sodB (R² = 0.57, N = 1089) and (F) shiA (R² = 0.26, N = 908); stability of DsrA SL3 and regulation of (I) hns (R² = 0.43, N = 2746) and (J) rpoS (R² = 0.33, N = 2655). Variants carrying the mutation A30G are outlined with black circles in panel (C), and an arrow indicates m8, the variant that carries this mutation alone.