Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2014 Sep 26;42(19):11879–11890. doi: 10.1093/nar/gku873

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2014. Published by Oxford University Press on behalf of Nucleic Acids Research.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.

PMC Copyright notice

Figure 7. — The PXDLS-containing proteins, NRIP1 and CBP, affect circadian rhythmicity in a PXDLS-dependent manner. Schematic representation of the evolutionary conservation of the PXDLS motif (green) in (A) NRIP1 and (B) CBP. NIH3T3 cells were transiently transfected with Bmal1-luciferase reporter either with control empty vector (black) or with (C) wild-type NRIP1-V5 (red) or PXDLS mutant NRIP1-V5 (green), (D) wild-type CBP-HA (Red) or PXDLS mutant CBP-HA (green). Cells were synchronized with a short dexamethasone (Dex) treatment, and real-time bioluminescence recording was performed using the LumiCycle. Amplitudes were quantified using the LumiCycle software. Data are presented on a bar graph as fold change (mean +/− SD, n = 4), P-values < 0.001 is marked with ** and <0.005 is marked with *. The expression levels of (E) wild-type NRIP1-V5 and PXDLS mutant NRIP1-V5 and (F) wild-type CBP-HA and PXDLS mutant CBP-HA were determined by SDS-PAGE and IB. U2AF was used as loading control. Molecular Weight (MW).