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. 2014 Sep 30;42(19):11952–11964. doi: 10.1093/nar/gku874

Figure 4.

Figure 4.

Effect of PTCAF-1 silencing on scnRNAs. (A) Distribution of life cycle stages in the Paramecium culture samples used for smallRNA libraries. RNA of an early and a late time point of a PTCAF-1 KD and a control culture was extracted and smallRNA libraries were prepared. Early time point samples contain cells in the vegetative (blue), skein (red) and fragmented (green) stage. Late time points contain cells in the fragmented (green) or late fragmented stage with visible developing MACs (purple). (B) Distribution of total 16 to 34 nucleotide small RNA library reads (Total/grey), mapped to the macronuclear genome (MAC/green), internal eliminated sequences (IES/red) and the plasmid backbone used for silencing (Vector/purple) (the same pair of histograms was previously used as the control in (28). (C) Effect of PTCAF-1 silencing on 25-nt scnRNA. Extraction of 25-nt reads mapping to MAC (green) and to IESs (red). (D) 25-nt scnRNAs reads mapped to the 51G locus. An extract of the 51G gene locus (green) containing three mcIES (red) and three non-mcIES (blue) was mapped with 25-nt scnRNA reads (black) from the early and late time point of PTCAF-1 silenced and control culture. The reads are mirrored over the corresponding sequence.