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. 2014 Sep 27;42(19):12102–12111. doi: 10.1093/nar/gku879

Figure 3.

Figure 3.

Primer extension activities of human PrimPol and PrimPolY89D. (A) Human PrimPol has primer extension activity and produces full-length templates following incubation for up to 60 min (lane 1 is a control and lanes 2–6 represent time points at 0.5, 1, 3, 5 and 60 min). (B) PrimPolY89D shows a marked reduction in polymerase activity, incorporating up to eight nucleotides following incubation for 60 min. (C) PrimPolY89S also shows a loss in polymerase activity relative to wild-type PrimPol, though it is a more active polymerase than PrimPolY89D. (D) PrimPolY89F restores polymerase activity. The Y89F variant can fully extend primers but there is an increased level of unextended primer relative to wild-type PrimPol.