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. 2014 Sep 27;42(19):12102–12111. doi: 10.1093/nar/gku879

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© The Author(s) 2014. Published by Oxford University Press on behalf of Nucleic Acids Research.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 8. — PrimPol Y89D cannot fully complement the UV sensitivity seen in PrimPol^−/− DT40 cells due to decreased replication fork speeds. (A) Western blot showing overexpression of wild type and Y89D point mutant forms of PrimPol in PrimPol knockout (PrimPol^−/−) DT40 chicken cells. (B) Cell viability was measured using CellTiter-Blue 48 h after increasing doses of UV-C were administered to the different cell types. Results represent three independent repeats and the error bars represent the standard deviation across the repeats. (C) DNA fibres were measured after sequential labelling with CldU and IdU for 20 min each to calculate replication speed. Results represent three independent repeats. (D) A 20 J/m² UV-C pulse was included between the two labels and the ratio of the pre- and post-UV-C labels was calculated, with increased ratios representing replication stalling after UV. Results represent three independent repeats.