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. 2014 Oct 1;42(19):11921–11927. doi: 10.1093/nar/gku896

Figure 4.

Figure 4.

Dynamics of DNA bound by H-NS and the mutant Hha(R14A/R17A). The black curves display the RMS excursion of the tethered microspheres as a function of time after release from the optical trap. The red curves are a control absent an applied force. Both proteins are at a concentration of 200 nM. The prominent solid curves are averaged over the respective trajectories for each population. All trajectories have been smoothed by a 60 s running window. While the mutant protein tends to reduce the RMS observed, the protein–DNA complex can no longer be triggered to collapse.